![]() This often results in loss of resolution in publication. If your figure is not conformed to the specified dimensions, the journal's typesetter will have to resize it. Figure dimensionsįigure dimensions are defined by typesetting format for the final PDF (or physical) print, and journal-specific. In general, most journals prefer vector files for line drawings (e.g., graphs) because they can ensure the highest quality and produce the best results in publication. To work with a vector file, you are probably using Adobe Illustrator, Adobe InDesign, Microsoft PowerPoint, or Inkscape (there are others as well). Vector images are object-based files like graphs created in Microsoft Excel and GraphPad Prism, and these images are supported by file formats including EPS, PDF, SVZ, PPTX, and AI. To edit a raster file, you would be using tools like Fiji/ImageJ, Adobe Photoshop, and GIMP (among others). Raster images are pixel-based files like EM images, and they include formats like TIFF, PNG, JPEG, BMP, and PSD. There are two categories of digital artwork: Raster and Vector images. RGB color or grayscale at 300 dpi monochrome at 1200 dpi The migration of protein molecular weight size markers or nucleic acid size markers must be indicated and labeled appropriately (e.g., “kD”, “nt”, “bp”) on all figure panels showing gel electrophoresis." All labels used in a figure should be explained in the legend. Do not include any two-bar graphs/histograms instead state those values in the text.Īll illustrations documenting results must include a bar to indicate the scale. Remove top and right borderlines that to not contain measuring metrics from all graph/histogram figure panels (i.e., do not box the panels in). ![]() For figures in vector-based format, all fonts should be converted to outlines and saved as EPS files to ensure that they are reproduced correctly. (← MK note: they are talking about graphs and line drawings in raster file format here.) Grayscale must be supplied at a minimum of 300 dpi. Monochrome (bitmap) images must be supplied at 1200 dpi. For line drawings that require color, consider redundant coding by adding different textures or line types to the colors.Ĭolor figures should be in RGB format and supplied at a minimum of 300 dpi. If no suitable combination can be found, consider presenting separate monochrome images for the different color channels. Figures with red and green are particularly problematic, and should generally be converted to magenta and green. Use textures or different line types rather than colors in bar plots or graphs. Grayscale generally provides a more faithful representation when a single quantity is displayed. Illustrations should be prepared so that they are accessible to our many color-blind readers, so color should only be used if it is necessary to accurately convey the information being presented by the image. They should be the smallest size that will convey the essential scientific information. A legend must be included in the manuscript document after the reference list, and should include enough detail to be intelligible without reference to the text.įigures must be submitted as separate files in TIFF or EPS format and be submitted at the size they are to appear: 1 column (maximum width 8.5 cm), 1.5 columns (maximum width 11.6 cm) or 2 columns (maximum width 17.6 cm). Do not duplicate data by presenting it both in the text and in a figure.Ī title should be part of the legend and not lettered onto the figure. Figures must be numbered independently of tables, multimedia, and 3D models and cited in the text.
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